Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multimodal AI biomarker PATH-ORACLE improves prediction of recurrence in stage I lung adenocarcinoma.

medRxiv : the preprint server for health sciences·2026
Same author

Silent and Deadly: Incidentally Discovered Asymptomatic Left Ventricular Pseudoaneurysm.

CASE (Philadelphia, Pa.)·2026
Same author

Impact of cognitive behavioral therapy on attention in patients with fibromyalgia: a randomized controlled trial.

Pain reports·2025
Same author

Pediatric virtual fracture clinic. Our first 10K!

Injury·2025
Same author

Tracking hemopexin intracellularly and defining hemopexin protein "interactomes" in human immune and liver cell models.

Frontiers in physiology·2025
Same author

Somatic evolution following cancer treatment in normal tissue.

Nature·2025
Same journal

Synthesis of tetrazole based donor-acceptor type fluorescent molecules and their interaction with transport protein bovine serum albumin.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Rational design of an S-scheme 2D/3D ZnIn<sub>2</sub>S<sub>4</sub>/K<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> heterojunction for enhanced photocatalytic antibiotic degradation.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Chlorin e6-mediated sublethal photodynamic therapy modulates biomarkers in a murine lung cancer-derived in vitro cancer-associated fibroblast model.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Polyethyleneimine-protoporphyrin IX and its Zn(II) complex conjugates for photodynamic inactivation of Staphylococcus aureus on cucumber mesocarp surfaces.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Canopy chlorophyll fluorescence escape fraction under polychromatic irradiation.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Genomic analysis highlights a novel class of DPCF-type cyanobacteriochrome.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
See all related articles

Related Experiment Video

Updated: May 22, 2026

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
11:04

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Published on: January 13, 2023

Stem cell-based photodynamic therapy.

Tej B Shrestha1, Gwi M Seo, Matthew T Basel

  • 1Kansas State University, Anatomy & Physiology, Manhattan, KS 66506, USA. tbs3@vet.k-state.edu

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
|May 9, 2012
PubMed
Summary
This summary is machine-generated.

This study engineered stem cells to secrete gaussia luciferase (gLuc) for photodynamic therapy (PDT). Coelenterazine-mediated PDT significantly reduced melanoma cells in vitro and showed a trend toward reduced tumor growth in vivo.

More Related Videos

Photodynamic Therapy with Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers
09:45

Photodynamic Therapy with Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers

Published on: October 28, 2015

An In Vitro Approach to Photodynamic Therapy
04:53

An In Vitro Approach to Photodynamic Therapy

Published on: August 17, 2018

Related Experiment Videos

Last Updated: May 22, 2026

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
11:04

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Published on: January 13, 2023

Photodynamic Therapy with Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers
09:45

Photodynamic Therapy with Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers

Published on: October 28, 2015

An In Vitro Approach to Photodynamic Therapy
04:53

An In Vitro Approach to Photodynamic Therapy

Published on: August 17, 2018

Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Stem Cell Biology

Background:

  • Photodynamic therapy (PDT) offers a targeted approach for cancer treatment.
  • Developing novel methods for PDT delivery and activation is crucial for improving efficacy.
  • Stem cells engineered for therapeutic protein secretion present a promising avenue for localized treatment.

Purpose of the Study:

  • To develop a novel stem cell-based system for delivering and activating photodynamic therapy.
  • To investigate the efficacy of engineered neural stem cells (NSCs) and rat umbilical cord matrix-derived stem cells (RUCMSCs) secreting gaussia luciferase (gLuc) in photodynamic therapy of melanoma.
  • To evaluate the in vitro and in vivo effects of coelenterazine-mediated PDT using gLuc-expressing stem cells.

Main Methods:

  • Murine neural stem cells (NSCs) and rat umbilical cord matrix-derived stem cells (RUCMSCs) were transfected with a gaussia luciferase (gLuc) expression plasmid.
  • gLuc-secreting stem cell supernatant was used for in vitro photodynamic therapy of murine melanoma cells (B16F10).
  • gLuc-expressing RUCMSCs were used for in vivo photodynamic therapy of lung melanomas in C57BL/6 mice, utilizing aminolevulic acid, gLuc, and coelenterazine.

Main Results:

  • A significant reduction in the viability of murine melanoma cells was observed in vitro.
  • A borderline significant retardation of tumor growth was noted in vivo following coelenterazine-mediated PDT.
  • The study demonstrated the feasibility of using gLuc-expressing stem cells for PDT delivery.

Conclusions:

  • Engineered stem cells secreting gaussia luciferase can be utilized for coelenterazine-mediated photodynamic therapy.
  • This approach shows potential for reducing melanoma cell viability in vitro and offers a basis for further in vivo investigation.
  • Further research is warranted to optimize this stem cell-based PDT system for enhanced therapeutic outcomes.