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Updated: May 22, 2026
The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
Published on: November 13, 2013
Histological analysis of the embryonic and adult tooth
1Department of Craniofacial Development, Dental Institute, King's College London, London, UK. atsushi.2.ohazama@kcl.ac.uk
Abstract:
Histology is the study of the microscopic anatomy of tissues by examining a thin slice of the tissue under the microscope. Prior to slicing/sectioning, most tissues require some form of solidifying to allow thin sections to be cut. However, since the tooth is the hardest substance in the vertebrate body, it is one of the most difficult tissues to process for histology. This chapter describes the methods used for making histological sections of tooth from different embryonic stages through to adulthood.
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