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Updated: May 22, 2026
Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
Published on: December 16, 2014
Detection of apoptosis by TUNEL assay
Kateryna Kyrylkova1, Sergiy Kyryachenko, Mark Leid
1Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.
Abstract:
Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.
