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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: May 22, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Potential pitfalls in microRNA profiling.

Pauline Chugh1, Dirk P Dittmer

  • 1Department of Microbiology, UNC-Chapel Hill, Chapel Hill, NC, USA.

Wiley Interdisciplinary Reviews. RNA
|May 9, 2012
PubMed
Summary
This summary is machine-generated.

MicroRNA (miRNA) profiling is crucial for understanding disease signatures and cellular processes. This review examines current miRNA expression profiling methods, highlighting their advantages and potential pitfalls for accurate analysis.

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Published on: August 21, 2019

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are small, noncoding RNAs regulating gene expression post-transcriptionally.
  • miRNAs impact diverse cellular functions, including immune response, cell cycle, migration, and apoptosis.
  • miRNA profiling is increasingly vital for identifying disease signatures.

Purpose of the Study:

  • To review current methods for miRNA expression profiling.
  • To discuss the advantages and potential pitfalls of these techniques.
  • To address key considerations in miRNA analysis.

Main Methods:

  • Comparative analysis of different miRNA profiling platforms.
  • Discussion of normalization strategies for miRNA expression data.
  • Evaluation of sample preparation and experimental analysis techniques.

Main Results:

  • Identification of advantages and limitations associated with various miRNA profiling methods.
  • Exploration of the impact of isomiRNAs on expression profiling accuracy.
  • Assessment of platform-specific variations and normalization challenges.

Conclusions:

  • Accurate miRNA profiling requires careful consideration of methodology, normalization, and sample handling.
  • Understanding the nuances of different profiling techniques is essential for reliable disease signature identification.
  • Further research is needed to optimize miRNA expression analysis for clinical applications.