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Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
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Spatial-temporal dynamics of collective chemosensing.

Bo Sun1, Josephine Lembong, Valery Normand

  • 1Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA.

Proceedings of the National Academy of Sciences of the United States of America
|May 9, 2012
PubMed
Summary

Multicellular organisms show regulated cell responses through intercellular communication and pacemaker cells. Gap junctions are essential for synchronized fibroblast cell calcium dynamics in dense colonies.

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Area of Science:

  • Cellular Biology
  • Biophysics
  • Systems Biology

Background:

  • Individual cell chemosensing is stochastic.
  • Multicellular organisms display regulated responses to stimuli.
  • Intercellular communication and pacemaker cells are key to deterministic chemosensing.

Purpose of the Study:

  • Characterize collective cell behavior in response to ATP stimulation.
  • Investigate the role of intercellular communication and pacemaker cells in spatial-temporal calcium dynamics.
  • Determine the impact of gap junctions on collective cell responses.

Main Methods:

  • Studied spatial-temporal calcium dynamics of fibroblast cells.
  • Compared responses of isolated cells, densely packed colonies, and cells in hydrogel films.
  • Analyzed the influence of gap junctions and diffusion-based communication.

Main Results:

  • Densely packed fibroblast colonies showed faster, synchronized, and correlated calcium responses compared to isolated cells.
  • Pacemaker cells were identified, and gap junctions were shown to influence collective responses.
  • Gap junctions are necessary for synchronized responses in high-density colonies; diffusion alone is insufficient.
  • Both gap junctions and diffusion enabled calcium oscillations, but hydrogel-trapped cells exhibited persistent oscillations.

Conclusions:

  • Intercellular signaling is crucial for regulated spatial and temporal dynamics in cell colonies and tissues.
  • Gap junctions play a vital role in coordinating responses in dense cell populations.
  • Cellular communication mechanisms dictate the nature and persistence of calcium dynamics.