Related Experiment Video
Updated: May 22, 2026
12:48
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
Published on: August 21, 2017
Cross-presentation: how to get there - or how to get the ER
Christoph Kreer1, Judith Rauen, Matthias Zehner
1Life and Medical Sciences Institute, University of Bonn Bonn, Germany.
Frontiers in Immunology
|May 9, 2012
Summary
Dendritic cells (DCs) use antigen cross-presentation to display external antigens on MHC I molecules, crucial for immunity and tolerance. This process involves specific endosomal compartments and ER-associated degradation machinery for antigen processing and presentation.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Antigen cross-presentation by dendritic cells (DCs) is vital for immune responses and tolerance.
- Extracellular antigens are internalized and protected in endosomal compartments for MHC I loading.
Purpose of the Study:
- To review recent advances in the molecular mechanisms of antigen cross-presentation.
- To highlight the role of specific endosomal compartments and ER-associated machinery in this process.
Main Methods:
- Review of current literature on antigen cross-presentation.
- Focus on endosomal targeting, antigen processing, and machinery recruitment.
Main Results:
- Antigen processing for cross-presentation involves cytoplasmic transport and re-import into endosomes.
- ER-associated degradation (ERAD) machinery and MHC I loading complex are recruited to endosomes.
- Specific endocytic receptors target antigens to protective endosomal compartments.
Conclusions:
- Antigen storage compartments are essential for cross-presentation.
- Molecular mechanisms involve ERAD and MHC I loading complex recruitment to endosomes.
- Cross-presentation shares parallels with endogenous antigen presentation via MHC I.
More Related Videos
Related Concept Videos
01:45ER Retrieval Pathway
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
01:34Crossing Over
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
01:30Crossing Over
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...
01:34Crossing over
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
01:28Protein Translocation Machinery on the ER Membrane
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
01:26Endoscopic Procedures V: ERCP
Endoscopic Retrograde Cholangiopancreatography (ERCP) is a diagnostic procedure that combines endoscopy and fluoroscopy to diagnose and treat conditions related to the bile ducts, pancreatic ducts, and gallbladder. This procedure is beneficial for identifying and addressing blockages, gallstones, strictures, and tumors within the biliary or pancreatic systems. ERCP is both diagnostic and therapeutic, offering the ability to visualize and treat identified problems in one session.
Patient...
Patient...