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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 22, 2026

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

An integrated, multiparametric flow cytometry chip using "microfluidic drifting" based three-dimensional hydrodynamic

Xiaole Mao, Ahmad Ahsan Nawaz, Sz-Chin Steven Lin

    Biomicrofluidics
    |May 9, 2012
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a miniature flow cytometry device for multi-parametric particle analysis. This integrated, single-layer chip offers comparable performance to traditional systems, enabling low-cost diagnostics.

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    Last Updated: May 22, 2026

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    The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

    Published on: April 22, 2014

    Area of Science:

    • Biomedical Engineering
    • Microfluidics
    • Optical Sensing

    Background:

    • Traditional flow cytometry systems are bulky, expensive, and complex.
    • Miniaturization is crucial for developing portable and accessible diagnostic tools.
    • Integrating multiple functions onto a single chip presents significant engineering challenges.

    Purpose of the Study:

    • To demonstrate an integrated, single-layer, miniature flow cytometry device.
    • To achieve multi-parametric particle analysis on-chip.
    • To advance low-cost flow cytometry for point-of-care applications.

    Main Methods:

    • Developed a microfluidic drifting-based three-dimensional (3D) hydrodynamic focusing component.
    • Integrated optical fibers directly into the microfluidic architecture for on-chip detection.
    • Simultaneously detected multiple optical signals (forward scatter, side scatter, fluorescence) from individual particles.

    Main Results:

    • The miniature flow cytometry chip successfully performed multi-parametric particle analysis.
    • On-chip detection of forward scatter, side scatter, and fluorescence was achieved.
    • The device's performance was found to be comparable to conventional desktop flow cytometers.

    Conclusions:

    • The integrated, single-layer microfluidic device enables on-chip 3D particle focusing and multi-parametric optical detection.
    • This technology represents a significant advancement towards mass-producible, low-cost flow cytometry chips.
    • The developed device holds promise for point-of-care clinical diagnostics.