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Updated: May 22, 2026

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
Published on: June 5, 2014
M. tuberculosis in Lymph Node Biopsy Paraffin-Embedded Sections
Abdurehman Eshete1, Ahmed Zeyinudin, Solomon Ali
1Department of Medical Laboratory Sciences and Pathology, College of Public Health and Medical Sciences, Jimma University, 409 Jimma, Ethiopia.
Tuberculosis lymphadenitis, a common extrapulmonary form, was evaluated in lymph node biopsies. The study found Mycobacterium tuberculosis in over 60% of biopsies, with higher prevalence in females, highlighting a need for targeted prevention.
Area of Science:
- Medical Microbiology
- Pathology
- Public Health
Background:
- Tuberculosis lymphadenitis is a prevalent form of extrapulmonary tuberculosis.
- Accurate diagnosis is crucial for patient management and disease control.
Purpose of the Study:
- To determine the prevalence of Mycobacterium tuberculosis in lymph node biopsies.
- To assess the utility of histological examination for diagnosing tuberculosis lymphadenitis.
Main Methods:
- A cross-sectional study utilizing histological examination of lymph node biopsy paraffin-embedded sections.
- Ziehl-Neelsen (ZN) and hematoxylin/eosin (H&E) staining techniques were employed.
- Study conducted at Jimma University Specialized Hospital from December 2009 to October 2010.
Main Results:
- Granulomas were observed in histopathological examinations.
- Caseation and necrosis were present in 85% of nodal tissue biopsies.
- Acid-fast bacilli (ZN staining) were confirmed in 61.7% of biopsies, with 56.7% of positive cases occurring in females.
Conclusions:
- Tuberculosis lymphadenitis is significantly more common in females.
- Histological examination, particularly ZN staining, is effective in diagnosing tuberculosis lymphadenitis.
- Control and prevention strategies should focus on extrapulmonary tuberculosis, especially in female populations.
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