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Updated: May 22, 2026

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae
Published on: June 25, 2018
[Thermal preference and avoidance in cladoceran Daphnia magna strauss (Crustacea, Cladocera) acclimated to constant
Abstract:
The final preferable temperature (FPT) and avoidance temperature (AT) were determined in parthenogenetic females of the crustacean Daphnia magna Strauss. The animals were preliminary acclimated to constant temperature of 23.4 degrees C followed by keeping them in a thermo-gradient device for 24 days. It was revealed that daphnia select FPT with overshoot. In the first four days, daphnia selected temperatures 0.6-1.6 degrees C higher than the acclimation temperature and 4-7.4 degrees C higher than FPT. Two zones of FPT are revealed: the first zone by the time of 5-13 days (17.6 +/- 1.2 degrees C); the second, by 16-24 days (20 +/- 1.5 degrees C). The dynamics of AT diapason followed the dynamics of FPT. Two zones of the AT plateau were observed: over five to 17 days (temperatures < 14 degrees C and > 25.8 degrees C were avoided) and for 21-24 days (< 8.5 degrees C and 26 degrees C).
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