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Updated: May 22, 2026

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
Published on: January 17, 2025
[The effect of nuclear dyes on the frequency of aberrations in mucosal cells of humans]
Abstract:
The effect of staining with commonly used nuclear dyes (orsein, light green, methylene blue, azure-eosin by Romanovsky-Giemsa) on the frequency of occurrence of nuclear disorders (micronuclei, perinuclear vacuoles, notches, protrusions, such as "tongue" and "broken egg") in human buccal mucosa was determined. It is shown that Romanovsky-Giemsa dye is the best type of dye both in the quality of the micropreparations and the spectrum of detected abnormalities.
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