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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: May 22, 2026

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
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Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

Published on: April 24, 2019

[Development of mRNA quantification after fluorescence activated cell sorting].

Hiroya Yamada1

  • 1Department of Hygiene, Fujita Health University School of Medicine, Toyoake 470-1192, Japan. hyamada@fujita-hu.ac.jp

Rinsho Byori. the Japanese Journal of Clinical Pathology
|May 10, 2012
PubMed
Summary
This summary is machine-generated.

A new method, mRNA quantification after fluorescence-activated cell sorting (FACS-mQ), enables the analysis of rare cell populations. This technique sorts cells by gene expression, overcoming limitations of traditional surface antigen methods for stem and cancer stem cells.

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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Context:

  • Detecting and analyzing rare cell populations, such as stem cells and cancer stem cells, is crucial in biomedical research.
  • Traditional methods like fluorescence-activated cell sorting (FACS) rely on surface antigens, which are often lacking in these critical cell types.
  • Maintaining cell viability during sorting is essential for subsequent biological characteristic analysis.

Purpose:

  • To introduce a novel laboratory test, mRNA quantification after fluorescence-activated cell sorting (FACS-mQ), designed to overcome the limitations of existing cell sorting techniques.
  • To enable the analysis of intracellular targets, including mRNA and nuclear antigens, in sorted cell populations.
  • To facilitate the study of cells that lack specific surface markers.

Summary:

  • FACS-mQ sorts cells based on specific gene expression patterns, allowing for the analysis of intracellular targets.
  • This method addresses the challenge of identifying and analyzing rare cells, particularly stem cells and cancer stem cells, which often lack unique surface antigens.
  • The procedure ensures cells remain viable for detailed biological characteristic analysis.

Impact:

  • FACS-mQ provides a powerful new tool for researchers studying human stem cells and cancer stem cells.
  • It is expected to significantly contribute to understanding the biology of rare and poorly characterized cell populations.
  • This advancement has the potential to accelerate discoveries in regenerative medicine and cancer research.