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Updated: May 22, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
[Finding targets of transcriptional regulators--chromatin immunoprecipitation assay (ChIP)]
Małgorzata Kus-Liśkiewicz1, Wiesława Widłak
1Biotechnology Department, Branch Campus of the Faculty of Biotechnology, Rzeszów University, Poland.
Abstract:
The protein-DNA interactions play a crucial role in many cellular processes such as DNA replication, recombination, repair, or gene transcription. Here we describe a chromatin immunoprecipitation (ChIP) technique, a tool for identifying DNA regions, which are in vivo associated with protein of interest. The main steps of ChIP are: fixation, chromatin fragmentation, immunoprecipitation with a specific antibody, and analysis of the immunoprecipitated DNA. Different analytical tools combined with ChIP (ChIP-chip, ChIP-seq) enable researchers to map protein binding in a truly genome-wide manner with high resolution.
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