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[Study on once sampling quantitation based on information entropy of ISSR amplified bands of Houttuynia cordata]
Haiqin Wang1, Wenlong Liu, Fuyuan He
1Pharmacey Bassic Teaching Experiment Center, School of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. wanghaiqingqing@163.com
Objective:
To explore the once sampling quantitation of Houttuynia cordata through its DNA polymorphic bands that carried information entropy, from other form that the expression of traditional Chinese medicine polymorphism, genetic polymorphism, of traditional Chinese medicine.
Method:
The technique of inter simple sequence repeat (ISSR) was applied to analyze genetic polymorphism of H. cordata samples from the same GAP producing area, the DNA genetic bands were transformed its into the information entropy, and the minimum once sampling quantitation with the mathematical mode was measured.
Result:
One hundred and thirty-four DNA bands were obtained by using 9 screened ISSR primers to amplify from 46 strains DNA samples of H. cordata from the same GAP, the information entropy was H=0.365 6-0.978 6, and RSD was 14.75%. The once sampling quantitation was W=11.22 kg (863 strains).
Conclusion:
The "once minimum sampling quantitation" were calculated from the angle of the genetic polymorphism of H. cordata, and a great differences between this volume and the amount from the angle of fingerprint were found.
