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Updated: May 22, 2026
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
Published on: October 21, 2010
[Study on andrographolide-induced apoptosis of Candida albicans biofilm dispersion cells]
Changzhong Wang1, Ning Han, Zhenhua Xu
1School of Integrated Traditional and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China. ahwcz63@sina.com
Objective:
To detect the effect of andrographolide on apoptosis of Candida albicans biofilm dispersion cells.
Method:
The morphological changes of apoptotic C. albicans biofilm cells were observed by using Hoechst 33258 staining Fluorescence microscope; changes of mitochondrial membrane potential (MMP) of C. albicans biofilm cells were detected by rhodamine 123 staining flow cytometry; and reactive oxygen species (ROS) was detected by DHR staining flow cytometry.
Result:
1 000, 100 micromol x L(-1) of andrographolide could cause pyknosis and dense staining of C. albicans biofilm cells, 1 000, 100, 10 micromol x L(-1) of andrographolide could decrease MMP and increase ROS of C. albicans biofilm cells.
Conclusion:
Andrographolide of appropriate concentrations could induce apoptosis of dispersion cells of C. albicans biofilms.
