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Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
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Human bocavirus types 1, 2 and 3 in acute gastroenteritis of childhood

Minna Risku1, Minna Kätkä, Suvi Lappalainen

  • 1Vaccine Research Center, Tampere University School of Medicine, Finland. minna.risku@uta.fi

Acta Paediatrica (Oslo, Norway : 1992)
|May 10, 2012
PubMed

Insights

Human bocavirus (HBoV) types 1, 2, and 3 were detected in children with acute gastroenteritis. HBoV2 was most common in single infections, suggesting a potential link to gastroenteritis requiring further study.

Area of Science:

  • Virology
  • Pediatrics
  • Infectious Diseases

Background:

  • Human bocavirus (HBoV) types 2 and 3 are recently identified and linked to childhood gastroenteritis.
  • Previous studies have investigated common viral gastroenteritis pathogens in children.

Purpose of the Study:

  • To investigate the prevalence of HBoV types 1, 2, 3, and 4 in children with acute gastroenteritis.
  • To determine if HBoV infections occur as single or mixed infections.

Main Methods:

  • Polymerase chain reaction (PCR) was used to detect HBoVs in 878 stool specimens from children with acute gastroenteritis and 112 controls.
  • Positive amplicons were sequenced to identify specific HBoV types (HBoV1, HBoV2, HBoV3, HBoV4).

Main Results:

  • HBoV was detected in 9.7% of gastroenteritis cases and 5.4% of controls.
  • HBoV1, HBoV2, and HBoV3 were identified; HBoV4 was not detected.
  • HBoV occurred as a single infection in 1.8% of cases, with HBoV2 being the most common type (50% of single infections).

Conclusions:

  • Human bocaviruses are infrequently detected as sole causative agents in pediatric acute gastroenteritis.
  • Further research is necessary to confirm a specific association between HBoV2 and gastroenteritis in children.
Abstract

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