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Updated: May 22, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
A highly selective and sensitive fluorescence "turn-on" probe for Ag+ in aqueous solution and live cells
Lin Xu1, Yufang Xu, Weiping Zhu
1Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
Abstract:
A naphthalimide-based fluorescent probe, NPQ, that contains a novel receptor was successfully developed. NPQ exhibited "turn-on" fluorescence and excellent selectivity toward Ag(+) in the presence of various other metal ions in aqueous solution. A series of control compounds were designed and synthesized in order to explore the photoinduced electron transfer (PET) quenching mechanism of NPQ and binding mode of NPQ with Ag(+). Moreover, with the NPQ-Ag(+) complex, I(-) was easily selectively recognized by a marked fluorescence quenching. The live cell imaging experiments demonstrate that NPQ can be used as a fluorescent probe for monitoring Ag(+) in living cells.
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