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Updated: May 22, 2026

Quantification of Hypopigmentation Activity In Vitro
Published on: March 6, 2019
Inhibitory effect of melanogenesis by 5-pentyl-2-furaldehyde isolated from Clitocybe sp
Young-Hee Kim1, Soo-Jin Choo, In-Ja Ryoo
1Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
Abstract:
In the continued search for melanogenesis inhibitors from microbial metabolites, we found that the culture broth of Clitocybe sp. MKACC 53267 inhibited melanogenesis in B16F10 melanoma cells. The active component was purified by solvent extraction, silica gel chromatography, Sephadex LH-20 column chromatography, and finally by preparative HPLC. Its structure was determined as 5- pentyl-2-furaldehyde on the basis of the UV, NMR, and MS spectroscopic analysis. The 5-pentyl-2-furaldehyde potently inhibited melanogenesis in B16F10 cells with an IC50 value of 8.4 microgram/ml, without cytotoxicity.
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