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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

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Published on: October 1, 2016

Fluorescence-based biosensors.

Maria Strianese1, Maria Staiano, Giuseppe Ruggiero

  • 1Department of Chemistry, University of Salerno, Salerno, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|May 11, 2012
PubMed
Summary
This summary is machine-generated.

This study reviews recent advancements in fluorescence-based optical sensors. Applications include monitoring hazardous gases and detecting celiac disease toxins like gliadin using advanced fluorescence techniques.

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Area of Science:

  • Optoelectronics and Photonics
  • Biomedical Engineering
  • Environmental Science

Background:

  • Optical sensors offer advantages over other transduction methods.
  • Fluorescence detection is a widely used and sensitive technique in optical sensing.
  • Combining fluorescence with ligand-binding proteins enhances selectivity in optical biosensors.

Purpose of the Study:

  • To review recent results on fluorescence-based sensors for environmental gas monitoring.
  • To discuss the implementation of optical sensors for detecting celiac disease-related proteins.
  • To explore various advanced optical sensing modalities.

Main Methods:

  • Fluorescence-based sensing for gas molecules (nitric oxide, hydrogen sulfide).
  • Reflectivity-based sensors.
  • Fluorescence correlation spectroscopy (FCS).
  • Enhanced fluorescence emission on silver island films (SIFs) coupled with total internal reflection fluorescence (TIRF) for protein detection.

Main Results:

  • Demonstrated implementation of fluorescence sensors for hazardous gas detection.
  • Showcased the use of SIFs and TIRF for sensitive detection of gliadin and prolamines.
  • Highlighted the versatility of fluorescence techniques in diverse sensing applications.

Conclusions:

  • Fluorescence-based optical sensors are effective for monitoring environmental gases.
  • Advanced optical techniques like SIFs/TIRF enable sensitive detection of foodborne toxins.
  • Optical sensors, particularly fluorescence-based ones, show significant potential in environmental and biomedical applications.