Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>N</i>-Methylation of Legionaminic Acid in <i>Legionella pneumophila</i> Serogroup 1 LPS Affects Cell Surface Properties, Intracellular Growth, and Cytokine Response.

Pathogens (Basel, Switzerland)·2026
Same author

Room-Temperature Luminescence of Eosin Y and Phloxine B in Red- to Near-Infrared Optical Region.

Journal of fluorescence·2026
Same author

Luminescence of N<sup>2</sup>,3-etheno-2-aminopurine Embedded in Polyvinyl Alcohol Films at Room Temperature.

Luminescence : the journal of biological and chemical luminescence·2026
Same author

Room temperature luminescence of a triangulenium dye ADOTA in PVA films.

Methods and applications in fluorescence·2026
Same author

Spectral properties of quinine sulfate in PVA films for front-face format emission measurements.

Methods and applications in fluorescence·2026
Same author

Substituents of the polysaccharide region of LPS in Legionella pneumophila sg1 modulate interactions with host cells.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026

Related Experiment Video

Updated: May 22, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
12:11

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Metal-enhanced immunoassays.

Ignacy Gryczynski1, Rafal Luchowski, Evgenia G Matveeva

  • 1Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, USA. igryczyn@hcs.unt.edu

Methods in Molecular Biology (Clifton, N.J.)
|May 11, 2012
PubMed
Summary
This summary is machine-generated.

Silver nanostructures enhance fluorescence detection in immunoassays. These structures reduce background noise and improve probe stability, leading to over 100-fold signal increases in specific applications.

More Related Videos

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
08:57

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

Published on: August 18, 2017

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
05:35

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Published on: January 17, 2020

Related Experiment Videos

Last Updated: May 22, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
12:11

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
08:57

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

Published on: August 18, 2017

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
05:35

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Published on: January 17, 2020

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Surface-confined assays are crucial for immunoassays but face challenges with fluorescence detection.
  • Background fluorescence from substrates can create artifacts and hinder accurate measurements.
  • Enhanced excitation and detection are needed for sensitive fluorescence-based assays.

Purpose of the Study:

  • To investigate the use of silver nanostructures for enhancing fluorescence detection in surface-confined immunoassays.
  • To evaluate the impact of silver island films (SIFs) and self-assembled colloidal structures (SACS) on fluorescence signals.
  • To assess the reduction of background fluorescence and improvement of probe photostability.

Main Methods:

  • Fabrication of SIFs and SACS on glass slide substrates.
  • Doping thin polymer layers with fluorescein dye for testing.
  • Characterization of fluorescence enhancement and background reduction using optical microscopy and spectroscopy.
  • Application of SACS surfaces in an Alexa488 model immunoassay.

Main Results:

  • Silver nanostructures significantly enhance measured fluorescence signals.
  • SACS surfaces demonstrated extraordinary fluorescence enhancements, exceeding 100-fold in hot spots.
  • Background fluorescence was substantially reduced by the silver nanostructures.
  • Improved photostability of fluorescent probes was observed.

Conclusions:

  • Silver nanostructures, particularly SACS, offer a powerful method for boosting fluorescence signals in surface-confined assays.
  • This approach addresses key limitations in immunoassay sensitivity and reliability.
  • The developed SACS-based surfaces show great promise for advanced diagnostic and detection platforms.