Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modified-Release Drug Delivery Systems: Site-Targeted

Modified-Release Drug Delivery Systems: Site-Targeted

Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

API particle size governs in situ forming implant formation, microstructure evolution and performance.

International journal of pharmaceutics·2026

Same author

Loteprednol etabonate nanostructured lipid carriers for enhanced ocular bioavailability.

Colloids and surfaces. B, Biointerfaces·2026

Same author

Spray freeze drying as a novel strategy to enhance oral delivery of proteins/peptides.

International journal of pharmaceutics·2026

Same author

Impact of macrophages on the dissolution of LAI suspension prodrugs.

International journal of pharmaceutics·2026

Same author

Insights into development of long-acting injectable suspensions.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

Zileuton nanocrystals alter intestinal phase I/II metabolic enzymes and epithelial permeability in a sex-dependent manner.

Drug metabolism and disposition: the biological fate of chemicals·2026

Search research articles

Related Experiment Video

Updated: May 22, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Efficient and safe delivery of siRNA using anionic lipids: Formulation optimization studies.

Mamta Kapoor¹, Diane J Burgess

¹Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, 69 N Eagleville Rd, Unit 3092, Storrs, CT 06269, USA.

International Journal of Pharmaceutics

|May 12, 2012

Summary

New anionic liposomes offer safe and effective small interfering RNA (siRNA) delivery for cancer therapy. These novel formulations achieve high gene silencing with minimal toxicity, presenting a safer alternative to cationic liposomes.

More Related Videos

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Related Experiment Videos

Last Updated: May 22, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Area of Science:

Biotechnology
Nanomedicine
Gene Therapy

Background:

Conventional cationic lipoplexes for siRNA delivery often exhibit significant cytotoxicity.
There is a need for safer and equally effective delivery systems for therapeutic small interfering RNA (siRNA).

Purpose of the Study:

To develop and optimize novel anionic lipoplexes for safe and efficient siRNA delivery in breast cancer cells.
To evaluate the efficacy, safety, and stability of anionic liposomes compared to cationic lipoplexes.

Main Methods:

Anionic liposomes (DOPG/DOPE) were complexed with siRNA using calcium ions to form anionic lipoplexes.
Formulation parameters including lipid composition, lipid and calcium concentrations were optimized.
Efficacy (gene silencing), cytotoxicity, stability in serum, cellular uptake, and endosomal escape were evaluated.

Main Results:

Optimized anionic lipoplexes (1μg/mL lipid, 2.4mM calcium, 10nM siRNA) achieved ~70% gene silencing with no cytotoxicity.
Anionic lipoplexes demonstrated stability in serum, efficient intracellular uptake, and endosomal escape.
Anionic formulations exhibited favorable characteristics: 324.2±19.6nm size, -22.9±0.1mV surface charge, and 98.5±1.4% encapsulation efficiency.

Conclusions:

Optimized anionic lipoplexes provide a safer alternative to cytotoxic cationic lipoplexes for siRNA delivery.
These formulations show potential for efficient in vitro and in vivo therapeutic siRNA delivery in cancer treatment.