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Updated: May 22, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
Published on: November 18, 2014
Synthetic peptide arrays for investigating protein interaction domains
Rudolf Volkmer1, Victor Tapia, Christiane Landgraf
1Institut für Medizinische Immunologie Berlin, Molecular Libraries and Recognition Group, Charité-Universitätsmedizin Berlin, Hessische Str. 3-4, 10115 Berlin, Germany. rve@charite.de
Abstract:
Synthetic peptide array technology was first developed in the early 1990s by Ronald Frank. Since then the technique has become a powerful tool for high throughput approaches in biology and biochemistry. Here, we focus on peptide arrays applied to investigate the binding specificity of protein interaction domains such as WW, SH3, and PDZ domains. We describe array-based methods used to reveal domain networks in yeast, and briefly review rules as well as ideas about the synthesis and application of peptide arrays. We also provide initial results of a study designed to investigate the nature and evolution of SH3 domain interaction networks in eukaryotes.
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