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A facile approach for sensitive, reversible and ratiometric detection of biothiols based on thymine-mediated

Boling Ma1, Fang Zeng, Xizhen Li

  • 1College of Materials Science & Engineering, South China University of Technology, Guangzhou 510640, P. R. China.

Chemical Communications (Cambridge, England)
|May 12, 2012
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...