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Nitric oxide is involved in hemin-induced cucumber adventitious rooting process
Wei Xuan1, Sheng Xu, Meiyue Li
1College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Journal of Plant Physiology
|May 15, 2012
Summary
Hemin promotes cucumber adventitious rooting by inducing heme oxygenase-1 (HO-1) and nitric oxide (NO) production, likely via a cyclic guanosine monophosphate (cGMP)-dependent pathway.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Hemin, a heme oxygenase-1 (HO-1) inducer, has beneficial physiological effects.
- HO-1/carbon monoxide (CO) signaling is implicated in auxin-induced adventitious rooting.
- The role of nitric oxide (NO) in hemin-induced rooting is unexplored.
Purpose of the Study:
- To investigate the involvement of nitric oxide (NO) in hemin-induced adventitious rooting in cucumber.
Main Methods:
- Cucumber explants were treated with hemin or CO.
- HO-1 transcripts (CsHO1), NO production, and root formation were analyzed.
- Specific inhibitors of nitric oxide synthase (NOS)-like enzymes, HO-1, NO scavengers, and guanylate cyclase inhibitors were used.
- Effects of tungstate (nitrate reductase inhibitor) and cyclic guanosine monophosphate (cGMP) were assessed.
Main Results:
- Hemin and CO up-regulated CsHO1 transcripts and NO production, promoting adventitious rooting.
- Inhibitors of NOS-like enzymes, HO-1, and NO scavengers blocked hemin-induced rooting.
- Tungstate did not affect hemin-induced rooting.
- Guanylate cyclase inhibitors reduced root development, while cGMP reversed this effect.
Conclusions:
- Nitric oxide (NO) acts downstream of hemin in promoting cucumber adventitious root formation.
- The process likely involves a cyclic guanosine monophosphate (cGMP)-dependent mechanism.
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