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EphB regulates L1 phosphorylation during retinocollicular mapping.

Jinxia Dai¹, Jasbir S Dalal, Sonal Thakar

¹Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA.

Molecular and Cellular Neurosciences

|May 15, 2012

Summary

Ephrin-B kinases phosphorylate the L1 cell adhesion molecule, modulating its interaction with ankyrin. This phosphorylation is crucial for the correct topographic mapping of retinal ganglion cell axons in the brain.

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Area of Science:

Neuroscience
Cell Biology
Molecular Biology

Background:

The cell adhesion molecule L1 is vital for the topographic mapping of retinal ganglion cell (RGC) axons to the superior colliculus (SC).
Abnormalities in L1 ankyrin-binding motifs (FIGQY(1229)H) lead to aberrant RGC axon mapping, similar to phenotypes observed in EphB receptor tyrosine kinase mutants.

Purpose of the Study:

To investigate the functional interaction between L1 and EphB receptors.
To determine if EphB kinases phosphorylate L1 at the ankyrin-binding site (FIGQY(1229)).

Main Methods:

Utilized HEK293 cells expressing L1 and EphB receptors to study phosphorylation.
Employed a phospho-specific antibody for tyrosine-phosphorylated FIGQY(1229) motif.
Generated L1-Y(1176)A knock-in mice and analyzed retinocollicular mapping.
Performed immunostaining and immunoblotting on mouse SC tissues.
Assessed neurite outgrowth in retinal explant cultures.

Main Results:

EphB2 kinase induced L1 phosphorylation at FIGQY(1229) and disrupted ankyrin recruitment in transfected cells.
Src family kinases mediated EphB2-induced L1 phosphorylation at FIGQY(1229).
EphB1 and EphB3 also mediated L1 phosphorylation at FIGQY(1229).
L1 phosphorylation at Tyrosine(1176) was unaffected by EphB2; L1-Y(1176)A mice showed normal mapping.
L1 colocalized with phospho-FIGQY in RGC axons within the SC.
L1 phosphorylation at FIGQY(1229) was reduced in EphB2/B3 mutant SC.
Inhibition of ankyrin binding in L1Y(1229)H mutant RGCs increased neurite outgrowth.

Conclusions:

EphB kinases phosphorylate L1 at FIGQY(1229), modulating L1-ankyrin binding.
This modulation is critical for the mediolateral retinocollicular topographic mapping.
L1-ankyrin binding appears to constrain RGC axon growth.