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Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Isobaric tagging approaches in quantitative proteomics: the ups and downs.

Andy L Christoforou1, Kathryn S Lilley

  • 1Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, Department of Biochemistry, University of Cambridge, Cambridge, UK. a.christoforou@gen.cam.ac.uk

Analytical and Bioanalytical Chemistry
|May 15, 2012
PubMed
Summary

Isobaric tagging is a popular quantitative proteomics method, but issues with accuracy and precision have emerged. This review discusses these challenges and proposes solutions for more reliable quantitative proteomics.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Published on: November 15, 2017

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Published on: May 1, 2017

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Quantitative Biology

Background:

  • Isobaric tagging is a widely adopted quantitative proteomics technique.
  • Its flexibility and multiplexing capabilities are significant advantages.
  • Rapid advancements in mass spectrometry have increased its application.

Purpose of the Study:

  • To review the challenges associated with isobaric tagging methods.
  • To discuss proposed solutions for improving quantitative accuracy and precision.
  • To enable quantitative proteomics at higher accuracy levels.

Main Methods:

  • Review of existing literature on isobaric tagging techniques.
  • Analysis of reported issues concerning quantitative accuracy and precision.
  • Discussion of emerging strategies and solutions.

Main Results:

  • Isobaric tagging methods face significant challenges in quantitative accuracy and precision.
  • These issues have become more apparent with improved mass spectrometer speed and sensitivity.
  • Various solutions are being explored to enhance the reliability of these methods.

Conclusions:

  • Addressing the limitations of isobaric tagging is crucial for advancing quantitative proteomics.
  • Improved accuracy and precision are necessary to meet the demands of biological research.
  • Ongoing research aims to refine these techniques for more robust data generation.