Related Experiment Video
Updated: May 22, 2026
09:31
Heterokaryon Technique for Analysis of Cell Type-specific Localization
Published on: March 11, 2011
The nucleolar detention pathway: A cellular strategy for regulating molecular networks
Timothy E Audas1, Mathieu D Jacob, Stephen Lee
1Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Cell Cycle (Georgetown, Tex.)
|May 15, 2012
Summary
A novel nucleolar detention pathway (NoDP) uses long noncoding RNA to immobilize proteins. This mechanism regulates molecular complex formation and downstream networks by controlling protein mobility.
Area of Science:
- Cellular Biology
- Molecular Biology
- Genetics
Background:
- Protein dynamics are crucial for timely molecular complex formation and self-assembly.
- Disruptions in protein availability can impair complex formation and downstream molecular networks.
- Efficient cellular function relies on precise regulation of molecular interactions.
Purpose of the Study:
- To identify and characterize a novel regulatory mechanism controlling protein mobility.
- To investigate the role of long noncoding RNA in modulating protein localization.
- To understand the implications of the nucleolar detention pathway (NoDP) in cellular regulation.
Main Methods:
- Inducible expression of novel long noncoding RNA.
- Investigating the nucleolar detention pathway (NoDP).
- Identifying putative targets of NoDP, such as DNMT1 and POLD1.
Main Results:
- A regulatory mechanism involving long noncoding RNA modulates protein mobility.
- The nucleolar detention pathway (NoDP) captures and immobilizes essential cellular factors in the nucleolus.
- NoDP targets include key proteins like DNMT1 and POLD1, suggesting a widespread regulatory role.
Conclusions:
- The nucleolar detention pathway (NoDP) represents a significant post-translational regulatory strategy.
- This pathway impacts molecular complex formation and downstream cellular networks.
- NoDP provides a mechanism to control protein availability and localization in response to environmental stimuli.
Related Concept Videos
01:45Regulation of Nuclear Protein Sorting
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
01:34Nuclear Protein Sorting
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
02:55The Nucleolus
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
02:55The Nucleolus
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
02:10Additional Subnuclear Structures
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals.
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...