A deletion in the nitrate high affinity transporter NRT2.1 alters metabolomic and transcriptomic responses to
Gemma Camañes1, Victoria Pastor, Miguel Cerezo
1Área de Fisiología Vegetal, Departamento de Ciencias Agrarias y del Medio Natural, ESTCE, Universitat Jaume I, Castellón, Spain.
Abstract:
A deletion in the high affinity nitrate trasporter NRT2.1 in Arabidopsis results in a reduced susceptibility to Pseudomonas syringae by two different mechanisms, the SA priming and an interference in the effector triggered susceptibility. In the present research we further characterized the metabolic and genetic profiles of the mutant nrt2 in the interaction with P. syringae. Despite the priming found in the SA-dependent pathway, the metabolic changes in nrt2 compared with wild-type plants are more remarkable prior infection. This is associated mainly to a pre-existing over representation of signals attributed to aromatic amino acids and phenylpropanoids in the nrt2. Genomic analysis confirms the implication of aromatic aminoacids and phenylpropanoids, but additionally, suggests a new role in ribosomal proteins as the major changes observed in nrt2 upon infection by the bacterium.
More Related Videos
08:34Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
Published on: January 8, 2020
07:35Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue
Published on: October 6, 2022
Related Concept Videos
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Stringent Response in E. coli
Inorganic Nitrogen Assimilation
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
Gene Regulation in Microbial Communities: Quorum Sensing
