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A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
Published on: March 3, 2016
Proteomics-based dissection of human endoderm progenitors by differential cell capture on antibody array
Revital Sharivkin1, Michael D Walker, Yoav Soen
1Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel.
Molecular & Cellular Proteomics : MCP
|May 15, 2012
Summary
Researchers developed a novel proteomics platform to analyze complex cell populations. This differential cell-capture antibody array effectively fractionates early human embryonic stem cell-derived endoderm, identifying distinct subpopulations for further study.
Area of Science:
- Developmental Biology
- Proteomics
- Stem Cell Biology
Background:
- Cellular heterogeneity and lack of specific markers hinder developmental mechanism studies.
- Early endoderm development from human embryonic stem cells is complex and poorly understood.
Purpose of the Study:
- To develop and validate a proteomics platform for dissecting heterogeneous cell populations.
- To fractionate and characterize subpopulations within the early endoderm derived from human embryonic stem cells.
Main Methods:
- Differential cell-capture antibody array: a high-throughput proteomics platform.
- Comparative screening of live cell populations using hundreds of cell-surface antigen antibodies.
- Fractionation of CXCR4+ early endoderm cells.
Main Results:
- Successfully fractionated the CXCR4+ early endoderm compartment.
- Identified distinct endoderm (CD61+, CD63+) and non-endoderm (CD271+, CD49F+, CD44+, B2M+) subpopulations.
- Characterized CD61+ cells as a direct derivative of CXCR4+ cells with unique emergence kinetics and gene expression.
Conclusions:
- The differential cell-capture antibody array is a powerful tool for detailed analysis of heterogeneous cellular systems.
- This platform enables the resolution of previously uncharacterized cell subpopulations in developmental studies.
- The findings provide new insights into early endoderm development and cell lineage tracing.
