Related Experiment Video
Updated: May 22, 2026
09:07
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Published on: May 1, 2019
HD2 proteins interact with RPD3-type histone deacetylases.
Ming Luo1, Yu-Yuan Wang, Xuncheng Liu
1Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
Plant Signaling & Behavior
|May 15, 2012
Summary
Plant-specific histone deacetylase HD2 proteins interact with RPD3-type histone deacetylases (HDACs) in Arabidopsis. This interaction suggests HD2 proteins and RPD3-type HDACs may form complexes to regulate plant gene expression.
Area of Science:
- Plant molecular biology
- Epigenetics
- Gene regulation
Background:
- Histone deacetylases (HDACs) play crucial roles in regulating gene expression.
- HD2 proteins are plant-specific HDACs, but their molecular functions remain largely unknown.
- Understanding HDAC function is key to deciphering plant epigenetic mechanisms.
Purpose of the Study:
- To investigate the molecular mechanism of HD2 protein function in plants.
- To determine if HD2 proteins interact with other known HDACs.
- To elucidate the potential role of HD2 proteins in gene expression regulation.
Main Methods:
- Bimolecular fluorescence complementation (BiFC) assay was employed.
- Interaction between specific Arabidopsis HD2 proteins (HD2A, HD2C, HD2D) and RPD3-type HDACs (HDA6, HDA19) was tested.
- Gene expression regulation was inferred from protein complex formation.
Main Results:
- Arabidopsis HD2 proteins (HD2A, HD2C, HD2D) were shown to interact with RPD3-type HDACs (HDA6 and HDA19).
- Evidence suggests that HD2 proteins and RPD3-type HDACs can form protein complexes.
- These findings provide molecular insights into the functional association of these proteins.
Conclusions:
- HD2 proteins likely function in conjunction with RPD3-type HDACs.
- The interaction indicates a potential role in a shared protein complex for gene regulation.
- This study advances our understanding of epigenetic regulation in plants mediated by HDACs.
Related Concept Videos
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
01:12The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
02:10The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
02:25Spreading of Chromatin Modifications
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Writers
The writer is an enzyme that can...