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06:39
Live Cell Imaging of Chromosome Segregation During Mitosis
Published on: March 14, 2018
A MatP-divisome interaction coordinates chromosome segregation with cell division in E. coli
Olivier Espéli1, Romain Borne, Pauline Dupaigne
1Centre de Génétique Moléculaire du CNRS, Associé à l'Université Paris-Sud, Gif-sur-Yvette, France. espeli@cgm.cnrs-gif.fr
The EMBO Journal
|May 15, 2012
Summary
Bacterial chromosome segregation is precisely controlled. The terminus (Ter) macrodomain (MD) movement to mid-cell relies on DNA replication and interaction with ZapB, revealing cell-cycle integration.
Area of Science:
- Microbiology
- Molecular Biology
- Cell Biology
Background:
- Bacterial chromosome segregation initiates near the origin of replication.
- The precise control mechanisms for the terminus region (Ter) of the Escherichia coli chromosome are less understood.
- MatP is a known structuring factor involved in Ter macrodomain (MD) organization.
Purpose of the Study:
- To elucidate the mechanisms controlling the segregation and localization of the Ter macrodomain (MD) in Escherichia coli.
- To understand the role of DNA replication and protein interactions in Ter MD positioning.
- To reveal how Ter MD segregation is integrated into cell-cycle control.
Main Methods:
- Characterization of Ter MD migration from the new pole to mid-cell.
- Analysis of Ter DNA replication dynamics and recruitment.
- Genetic manipulation of the chromosome to split the Ter MD.
- Investigation of MatP and ZapB interactions using mutant strains.
Main Results:
- Ter MD recruitment to mid-cell is sequential and correlated with Ter DNA replication.
- Replisomes are repositioned to mid-cell during Ter DNA replication, even in a split Ter MD system.
- Anchoring of the Ter MD at mid-cell is dependent on the MatP-ZapB interaction.
Conclusions:
- The segregation of the Ter MD is a tightly regulated process.
- DNA replication and the MatP-ZapB interaction are critical for Ter MD positioning at mid-cell.
- Ter MD segregation is integrated into the bacterial cell-cycle control.
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