Histopathological changes in Poecilia latipinna male gonad due to Tribulus terrestris administration

Tribulus terrestris is a traditionally known non-toxic aphrodisiac herb for maleness. It was experimented recently to understand the effect and mechanism on mono sex production in Poecilia latipinna. It would help to develop a new eco-friendly way to masculinize P. latipinna, since males have higher commercial value than females. The different concentration (100, 150, 200, 250 and 300 mg/L) of T. terrestris extract and a control were tested for their effect on sex transformation/reversal in P. latipinna by immersing the newly born young ones in the respective concentrations for 2 mo. The obtained results indicate that a dose dependant masculinization is obtained due to T. terrestris administration, which improved the male proportion. Histological results revealed that the testes of fish treated with T. terrestris extract contained all stages of spermatogenesis, clearly demonstrating that the administration of T. terrestris extract to P. latipinna stimulated spermatogenesis. Thus, it is discernible that 0-d-old hatchlings of P. latipinna exposed to T. terrestris extract orient/reverse their sex more towards maleness besides yielding better growth and spermatogenesis which is a mandate for fancy fish industry.