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Updated: May 22, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
Published on: April 12, 2015
MicroRNAs: Synthesis, Gene Regulation and Osteoblast Differentiation
1Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, Tamil Nadu, India.
Abstract:
The central dogma of transfer of genetic information from DNA to protein via mRNA is now challenged by small fragment of non coding RNAs typically 19-25 nucleotides in length namely microRNAs (miRNAs). miRNAs regulate expression of the protein coding genes by interfering in their mRNAs and, thus, act as key regulators of diverge cellular activities. Osteoblast differentiation, a key step in skeletal development involves activation of several signalling pathways including TGFb, BMP, Wnt and transcription factors, which are tightly regulated by miRNAs. In this review, we provide information on recent developments of the synthesis and gene regulation of miRNAs as well as the potential nature of miRNAs that regulate mesenchymal stem cell towards osteoblast differentiation for therapeutic purpose.
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