Lymphoid dendritic accessory cells of the rat

The expression of MHC class II antigens on potential APC is a crucial step in T-lymphocyte activation and the initiation of an immune response. The studies which are presented here were initiated to characterize the critical APC present in physiologically normal, untreated rats. Such a cell should constitutively express these antigens at high density and therefore provide the apparatus necessary to provoke both primary and secondary immune responses at any time. DAC were found to fulfill these criteria. In the absence of specific surface markers of rat DAC, the results are based on the strict combination of morphological appearance and functional activity. However, the high expression of MHC class II antigens may be regarded as semispecific markers for DAC which are distributed at strategic positions in many lymphoid and nonlymphoid tissues (Hart & Fabre 1981, Steiniger et al. 1984). The relatively low number of these cells observed in tissue sections and in in vitro isolates (0.1% of all cells) may explain their high activity as APC. This would facilitate the presentation of antigen in vivo to a sufficient number of competent T lymphocytes. DAC differentiate from a bone marrow progenitor cell pool preferentially under the influence of spleen cell-derived activities. Although the exact lineage has not yet been determined it may be fair to speculate that DAC form a new cell lineage probably related to interdigitating cells but not to macrophages which differentiate from bone marrow-derived precursors under the influence of colony-stimulating activities. However, the cooperation between DAC plus macrophages may provide the stage for T-lymphocyte activation and T-T collaboration (Mitchison 1990). There are still many open questions concerning the general role of DAC in vivo and in vitro. To further characterize rat DAC, their tissue distribution, role in the immune response and possible influence on intrathymic lymphopoiesis, with respect to T-lymphocyte subpopulations and the selection of the T-lymphocyte antigen-receptor repertoire, a panel of DAC-specific monoclonal antibodies must be generated in the future. Such antibodies will also be useful to study the mechanism by which DAC activate T lymphocytes.