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Na+ absorption by Claudius' cells is regulated by purinergic signaling in the cochlea
Jae Chul Yoo1, Hye-Young Kim, Kyu-Hee Han
1Department of Otorhinolaryngology, National Medical Center, Seoul, Korea.
Conclusion:
Claudius' cells absorb Na(+) through the amiloride-sensitive epithelial sodium channel (ENaC). Transepithelial ion transport through ENaC and possibly a Cl(-) secretory pathway is regulated by P2Y purinergic signaling.
Objectives:
The purpose of this study was to investigate ion transport in Claudius' cells and its purinergic regulation.
Methods:
Young adult Sprague-Dawley rats and gerbils were studied. The Claudius' cell layer on the basilar membrane was dissected from the basal turn of the cochlea. A voltage-sensitive vibrating probe was used to measure transepithelial short circuit current (I(sc) ). The baseline I(sc) of Claudius' cells was measured in the perilymph-like control solution and the change of I(sc) after application of amiloride (10 μM) or uridine triphosphate (UTP, 100 μM).
Results:
A negative baseline I(sc) was observed in the control solution (-12.50 ± 3.95 μA/cm(2), n = 8) and the addition of amiloride resulted in a decrease of I(sc) by 75.8%. The application of UTP, an agonist for P2Y purinergic receptors, led to a partial inhibition of I(sc) (by 38.2 ± 3.2%, n = 5), and subsequent addition of amiloride abolished the remaining I(sc).
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