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Significant decrease in maternal serum concentrations of angiopoietin-1 and -2 after delivery.

Elina Keikkala¹, Timo Hytinantti, Katja-Anneli Wathén

¹Department of Obstetrics and Gynecology, University of Helsinki, Helsinki University Central Hospital, Helsinki, Finland. elina.keikkala@hus.fi

Acta Obstetricia Et Gynecologica Scandinavica

|May 16, 2012

Summary

Maternal angiopoietin (Ang)-1 and Ang-2 levels decrease after delivery, while umbilical cord concentrations of Ang-1 and Tie-2 are elevated compared to maternal serum. This study characterizes these key angiogenic factors during childbirth.

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Area of Science:

Reproductive biology
Vascular biology
Perinatology

Background:

Angiopoietins (Ang)-1 and Ang-2, along with soluble endothelial cell-specific tyrosine kinase receptor-2 (Tie-2), are crucial regulators of angiogenesis and vascular stability.
Their precise physiological distribution and dynamic changes during term pregnancy and postpartum are not fully elucidated.

Purpose of the Study:

To characterize the physiological distribution of angiopoietins (Ang)-1 and Ang-2 and soluble Tie-2 (sTie-2) in maternal serum, umbilical cord serum, amniotic fluid, and urine at term and following delivery.
To investigate the changes in these factors after childbirth.

Main Methods:

A prospective, descriptive study was conducted with 20 healthy term pregnant women and their newborns undergoing elective cesarean delivery.
Concentrations of Ang-1, Ang-2, and Tie-2 were measured using enzyme-linked immunosorbent assay (ELISA) in maternal antepartum and postpartum serum, umbilical cord serum, amniotic fluid, and maternal and newborn urine.

Main Results:

Maternal serum concentrations of Ang-1 and Ang-2 significantly decreased after delivery (p < 0.017 and p < 0.0001, respectively).
Maternal serum Tie-2 concentrations remained stable postpartum (p=0.107).
Umbilical cord serum exhibited higher concentrations of Ang-1 (p < 0.0001) and Tie-2 (p < 0.0001) compared to maternal antepartum serum, with similar Ang-2 levels (p=0.067).
Low concentrations of Ang-1, Ang-2, and Tie-2 were found in amniotic fluid, and these factors were largely undetectable in maternal and newborn urine.

Conclusions:

Maternal levels of Ang-1 and Ang-2 decrease after delivery, suggesting a role in postpartum vascular remodeling.
Elevated umbilical cord concentrations of Ang-1 and Tie-2 indicate their significant contribution to fetal vascular development.