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Updated: May 22, 2026

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen
06:42

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

Published on: April 6, 2017

Liquid nitrogen cryosurgery for treatment of mucoceles in children

Paulo de Camargo Moraes1, Rubens Gonçalves Teixeira, Luiz Alexandre Thomaz

  • 1Department of Oral Surgery, in the School of Dentistry, São Leopoldo Mandic, Campinas, São Paulo, Brazil.

Pediatric Dentistry
|May 16, 2012
PubMed

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Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...