High-performance liquid chromatography of antibiotics

Area of Science:

• Analytical Chemistry
• Pharmacology
• Pharmaceutical Analysis

Background:

• High-performance liquid chromatography (HPLC) is increasingly utilized for antimicrobial agent monitoring.
• HPLC offers a valuable alternative to existing analytical methods due to its specificity and accuracy.
• The applicability of HPLC extends to nearly all classes of antibiotics.

Purpose of the Study:

• To outline the systematic development of HPLC methods for novel antibiotics.
• To address challenges in standardizing sample preparation and mobile phase development for antibiotic analysis.
• To present representative HPLC techniques for major antibiotic classes.

Main Methods:

• Review of established and emerging HPLC methodologies for antimicrobial quantification.
• Detailed examination of critical steps in HPLC method development, including sample preparation and mobile phase optimization.
• Case studies illustrating HPLC applications for diverse antibiotic categories.

Main Results:

• HPLC demonstrates high specificity and accuracy in antimicrobial monitoring.
• Standardization of sample preparation and mobile phase selection are key challenges addressed.
• Effective HPLC protocols are presented for various antibiotic classes.

Conclusions:

• HPLC is a robust and versatile technique for the analysis of antimicrobial agents.
• Standardized protocols are crucial for reliable HPLC method development and application.
• This review provides a comprehensive guide for utilizing HPLC in antibiotic analysis.