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Updated: May 22, 2026

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
Published on: February 7, 2022
Protein solubilization: attend to the choice of lysis buffer
Mandy Peach1, Noelle Marsh, Daniel J Macphee
1Memorial University of Newfoundland, St. John's, NL, Canada.
Abstract:
The efficient extraction of proteins of interest from cells and tissues is not always straightforward. In this process, the use of the optimal lysis buffer for protein solubilization should be considered. Here we demonstrate the use of a urea/thiourea lysis buffer, based on O'Farrell's buffer, and compare its effectiveness for solubilization of proteins from smooth muscle with the often utilized RIPA lysis buffer.
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