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Updated: May 22, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
A combined free flow electrophoresis and DIGE approach to compare proteins in complex biological samples
Kim Y C Fung1, Chris Cursaro, Tanya Lewanowitsch
1CSIRO, Preventative Health Flagship, Australia, Adelaide BC, SA, Australia. kim.fung@csiro.au
Abstract:
Free flow electrophoresis (FFE) has been applied in numerous studies as a protein separation technique due to its multiple advantages such as fast and efficient sample recovery, high resolving power, high reproducibility, and wide applicability to protein classes. As a stand-alone platform however, its utility in comparative proteomic analysis is limited as protein samples must be run sequentially rather than simultaneously which introduces inherent variability when attempting to perform quantitative analysis. Here we describe an approach combining fluorescent CyDye technology (DIGE) with FFE to simultaneously separate and identify differentially expressed proteins in a model cell system.
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