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Updated: May 22, 2026
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Native flatbed isoelectric focusing for determining antibody clonotype distribution
Biji T Kurien1, R Hal Scofield
1Department of Veterans Affairs, University of Oklahoma Health Sciences Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. biji-kurien@omrf.org
Abstract:
A sensitive method to analyze specific antibody clonotype changes in a patient with systemic lupus erythematosus, who developed autoantibodies to the Ro 60 autoantigen under observation, is described in this chapter. Patient sera collected over several years were separated by flatbed isoelectric focusing (IEF). Following electrofocusing, the gel is now ready to be analyzed by affinity immunoblotting utilizing Ro 60-coated nitrocellulose membrane to determine oligoclonality of the anti-Ro 60-containing sera.
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