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Updated: May 22, 2026

Western Blotting: Sample Preparation to Detection
07:45

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

A single-step simultaneous protein staining procedure for polyacrylamide gels and nitrocellulose membranes by Alta

Jayanta K Pal1, Sunil K Berwal, Rupali N Soni

  • 1University of Pune, Pune, India. jkpal@unipune.ac.in

Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2012
PubMed

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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
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