Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Acute Exposure to Perfluorooctanoic Acid (PFOA) During Cardiomyogenesis disrupts Transcriptional and Electrophysiological Profiles in Differentiated Myocytes.

bioRxiv : the preprint server for biology·2026

Same author

A new approach methodology for studying intrinsic ventricular arrhythmias in Fabry disease.

Frontiers in cardiovascular medicine·2026

Same author

Donor-matched iPSC model reveals context-dependent T2D genetic signals in fibro-adipogenic progenitors.

bioRxiv : the preprint server for biology·2026

Same author

EBF2 variant identified in a patient with atypical partial lipodystrophy causes adipose fibrosis and dysfunction.

The Journal of clinical investigation·2026

Same author

Generation and characterization of two human induced pluripotent stem cell lines from young and older male donors.

Stem cell research·2025

Same author

Developing an approach for evaluating the cardiotoxic potential of botanicals.

Frontiers in toxicology·2025

Search research articles

Related Experiment Video

Updated: May 22, 2026

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

Published on: October 19, 2021

Advances in embryo culture systems.

Gary D Smith¹, Andre Monteiro da Rocha

¹Department of Obstetrics and Gynecology University of Michigan, Ann Arbor, Michigan, USA. smithgd@umich.edu

Seminars in Reproductive Medicine

|May 16, 2012

Summary

Advances in mammalian embryo culture focus on optimizing the physical environment beyond just media. Innovations in insoluble culture, dynamic systems, and precise monitoring enhance embryo development and selection for improved assisted reproductive technologies.

More Related Videos

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

Published on: January 24, 2015

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Published on: June 12, 2020

Related Experiment Videos

Last Updated: May 22, 2026

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

Published on: October 19, 2021

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

Published on: January 24, 2015

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Published on: June 12, 2020

Area of Science:

Reproductive Biology
Developmental Biology
Biotechnology

Background:

Mammalian embryo culture has advanced significantly over 60-70 years.
Crucial for basic research on preimplantation development and clinical assisted reproductive technologies (ART).
Early focus on soluble media components improved embryo culture success.

Purpose of the Study:

To review recent advancements in preimplantation embryo culture.
Highlighting innovations beyond traditional media composition.
Focusing on improving embryo development and selection for ART.

Main Methods:

Modifying the insoluble culture environment.
Developing dynamic rather than static culture conditions.
Implementing precise gas phase and media pH regulation/monitoring systems.

Main Results:

Shift in focus from soluble media to the physical and dynamic aspects of the culture environment.
Integration of analytical platforms for embryo assessment.
Potential for improved selection of embryos with higher implantation potential.

Conclusions:

Recent innovations in preimplantation embryo culture extend beyond media formulation.
Dynamic and precisely regulated environments, coupled with advanced analysis, are key.
These advancements promise to further enhance the success of assisted reproductive technologies.