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Updated: May 22, 2026
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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Published on: July 26, 2017
Orai1, STIM1, and their associating partners
Sonal Srikanth1, Yousang Gwack
1Department of Physiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA. ssrikanth@mednet.ucla.edu
The Journal of Physiology
|May 16, 2012
Summary
Store-operated calcium (SOC) entry regulates intracellular calcium levels in non-excitable cells. This review details the molecular players, STIM1 and Orai1, in calcium release-activated calcium (CRAC) channels and their interactions.
Area of Science:
- Cellular Biology
- Molecular Biology
- Immunology
Background:
- Store-operated calcium (SOC) entry is crucial for regulating intracellular Ca2+ in non-excitable cells.
- Calcium release-activated calcium (CRAC) channels, a type of SOC channel, are well-studied in immune cells.
- The identification of STIM1 and Orai1 has significantly advanced the molecular understanding of CRAC channels.
Purpose of the Study:
- To review the current understanding of CRAC channel regulation.
- To explore the interacting partners of STIM1 and Orai1.
- To discuss the physiological roles of CRAC channels at organism and molecular levels.
Main Methods:
- Literature review of recent advancements in CRAC channel research.
- Analysis of studies utilizing transgenic animal models.
- Examination of electrophysiological and biochemical data.
Main Results:
- STIM1 acts as an endoplasmic reticulum Ca2+ sensor, while Orai1 forms the channel pore.
- Interacting partners of STIM1 and Orai1 are key in regulating CRAC channel activity.
- CRAC channels play significant physiological roles beyond immune cells.
Conclusions:
- The molecular machinery of CRAC channels is increasingly understood.
- Further research into STIM1/Orai1 interacting partners will elucidate broader physiological functions.
- CRAC channels are vital for cellular calcium homeostasis and function in diverse cell types.
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