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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

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Published on: September 3, 2013

Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice.

Stacy M Kenyon¹, Noah G Weiss, Mark A Hayes

¹Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.

Electrophoresis

|May 17, 2012

Summary

This study demonstrates a microscale device using electrophoretic exclusion to separate particles and molecules. The novel technique successfully differentiated between microspheres and dye molecules in solution.

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Area of Science:

Analytical Chemistry
Microfluidics
Separations Science

Background:

Electrophoretic exclusion is a technique that uses opposing electrophoretic and hydrodynamic forces to separate substances.
Adapting this technique to microscale devices offers potential for advanced analytical applications.

Purpose of the Study:

To adapt electrophoretic exclusion to a microscale device for species differentiation.
To demonstrate the device's capability in excluding and differentiating small particles and molecules.

Main Methods:

Development of a microscale device for electrophoretic exclusion.
Proof-of-principle experiments using 1 μm polystyrene microspheres and rhodamine 123 fluorescent dye.
Direct observation of electrophoretic exclusion behavior on the microchip.

Main Results:

The microscale device successfully excluded polystyrene microspheres and rhodamine 123 from a channel entrance.
Effective differentiation between the microspheres and dye molecules was achieved.
Demonstrated direct observation of the electrophoretic exclusion phenomenon at the microscale.

Conclusions:

Electrophoretic exclusion is effectively adapted to a microscale device.
The device shows promise for microscale separations and differentiation of particles and molecules.
Further studies will focus on optimizing and applying this microchip-based technique.