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Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
14:49

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

Construction of RNA-Seq libraries from large and microscopic tissues for the Illumina sequencing platform

Hagop S Atamian1, Isgouhi Kaloshian

  • 1Department of Nematology, Center for Disease Vector Research, Center for Plant Cell Biology, University of California, Riverside, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 17, 2012
PubMed

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...