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Related Concept Videos

Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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Published on: January 16, 2017

Polymethacrylate-based monolithic capillary column with weak cation exchange functionalities for capillary

Cemil Aydoğan1, Ali Tuncel, Adil Denizli

  • 1Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.

Journal of Separation Science
|May 17, 2012
PubMed
Summary

Researchers developed a novel polymethacrylate monolith for capillary electrochromatography (CEC) using an amino acid-based monomer. This weak cation exchange material enables effective separation of various compounds via cathodic electroosmotic flow.

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Last Updated: May 22, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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Published on: July 14, 2015

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Materials Science

Background:

  • Capillary electrochromatography (CEC) offers high-efficiency separations.
  • Developing novel stationary phases is crucial for advancing CEC.
  • Polymethacrylate monoliths provide a versatile platform for chromatographic applications.

Purpose of the Study:

  • To synthesize a novel polymethacrylate-based monolith with weak cation exchange functionalities.
  • To investigate its application in capillary electrochromatography (CEC).
  • To explore the separation of diverse analytes using this new material.

Main Methods:

  • In situ polymerization of butyl methacrylate, ethylene dimethacrylate, and N-methacryloyl-L-glutamic acid within a capillary column.
  • Preparation of the stationary phase involved monomer synthesis, capillary silanization, and polymerization.
  • Utilized a porogen mixture of N,N-dimethyl formamide and phosphate buffer.
  • Capillary electrochromatography (CEC) separations were performed using varying acetonitrile/buffer compositions and voltages.

Main Results:

  • Successfully prepared a polymethacrylate monolith with weak cation exchange properties in a capillary column.
  • Demonstrated effective CEC separations of alkyl benzenes, acidic, basic, phenolic, and polycyclic aromatic compounds.
  • Achieved separations using cathodic electroosmotic flow.

Conclusions:

  • The novel amino acid-based monomer (N-methacryloyl-L-glutamic acid) is suitable for creating functional monoliths for CEC.
  • The developed weak cation exchange monolith exhibits good performance for separating a wide range of analytes.
  • This approach offers a promising new stationary phase for capillary electrochromatography.