Biological cryosection preparation and practical ion yield evaluation for ion microscopic analysis
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E W Sod1, A R Crooker, G H Morrison
1Department of Chemistry, Cornell University, Ithaca, NY 14853.
Journal of Microscopy
|October 1, 1990
Abstract:
A mounting method utilizing an indium substrate is described for preparing freeze-dried cryosections of biological tissue for ion microscopic analysis. Using this procedure, a qualitative comparison between cryosection, conventional chemical, and freeze-substitution specimen preparations is made with rat liver tissue. Practical ion yield variations in cryosections are found to be minimal (+/- 13% relative standard deviation) in tissue-containing areas of rat liver and small intestine.