Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 22, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Digital microfluidic hydrogel microreactors for proteomics.

Vivienne N Luk1, Lindsey K Fiddes, Victoria M Luk

  • 1Department of Chemistry, University of Toronto, Toronto, ON, Canada.

Proteomics
|May 17, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A digital microfluidic platform for cell-based non-invasive testing.

iScience·2026
Same author

Correction: A microfluidic approach to evaluating surface protection from nonspecific antibody adsorption.

Lab on a chip·2026
Same author

Fibrillar Hydrogel Derived from Nanocellulose and a Synthetic Polypeptide.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

A microfluidic approach to evaluating surface protection from nonspecific antibody adsorption.

Lab on a chip·2026
Same author

Strain stiffening and compression-induced softening of composite fibrous hydrogels derived from rod-shaped nanoparticles and a synthetic copolymer.

Materials horizons·2026
Same author

DROP-LCMS for wastewater surveillance of viral disease.

Lab on a chip·2026
Same journal

Light-Induced Proteomic Changes in Pseudomonas aeruginosa Biofilms.

Proteomics·2026
Same journal

Decade-Resolved Proteomic Profiling of Gastric Cancer FFPE Archives: Evaluating Storage-Associated Shifts and Signal Stability Over 50 Years.

Proteomics·2026
Same journal

Proteome-Scale Mining of Metal-Associated Proteins of Monkeypox Virus.

Proteomics·2026
Same journal

Optimized Sample Handling Minimizes Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics.

Proteomics·2026
Same journal

Toward Predicting Pandemic Potential: A Comparative Analysis of Virus-Host Interactions Between Diverse Influenza A Viruses and the Human Innate Immune System.

Proteomics·2026
Same journal

Functional Divergence of Mucus in Pacific Oyster (Crassostrea gigas): Insights From Integrated Proteomic and Rheological Study.

Proteomics·2026
See all related articles

Immobilized enzymes in hydrogels integrated with digital microfluidic systems offer efficient proteomic sample processing. This approach enhances protein digestion and supports automation for miniaturized proteomics.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Proteolytic digestion is crucial for proteomic sample preparation.
  • Traditional methods use homogeneous enzyme solutions.
  • There is a trend towards heterogeneous systems with immobilized enzymes for improved efficiency and automation.

Purpose of the Study:

  • To introduce immobilized enzymes in hydrogels for proteomic sample processing within digital microfluidic (DMF) systems.
  • To optimize enzyme immobilization and digestion efficiency.
  • To demonstrate the utility of this approach for automated and miniaturized proteomics.

Main Methods:

  • Cylindrical agarose discs with immobilized trypsin or pepsin were integrated into DMF devices.
  • A fluorogenic assay was used to optimize enzyme loading (31 μg trypsin in 2-mm discs).

More Related Videos

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Related Experiment Videos

Last Updated: May 22, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

  • An integrated DMF method involved sequential reduction, alkylation, and digestion of proteomic samples.

    • Main Results:

    • Immobilized trypsin on agarose discs in DMF achieved higher sequence coverage compared to conventional homogeneous digestion.
    • The DMF droplet operation allowed precise control over sample and reagent handling.
    • Proof-of-principle for parallel digestion using multiple enzyme-bearing gel discs was demonstrated.

    Conclusions:

    • Immobilized enzymes in hydrogels within DMF systems provide an efficient and automatable method for proteomic sample processing.
    • This technique facilitates miniaturization and enhances digestion efficiency.
    • It represents a valuable tool for advancing automated proteomics workflows.