Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rotation function studies of human C-reactive protein.

D A Myles1, S A Rule, L J DeLucas

  • 1Department of Physics, University of Keele, Staffordshire, U.K.

Journal of Molecular Biology
|December 5, 1990
PubMed
Summary

Rotation function studies confirm the pentameric structure of human C-reactive protein. Dissociation heterogeneity limited reproducible crystal growth, but molecule orientation was consistent across two crystal forms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The 1.3-A Structure of a Scorpion Neurotoxin: A Membrane-binding Protein.

Biophysical journal·2009
Same author

Analysis of haematopoietic chimaerism by quantitative real-time polymerase chain reaction.

Bone marrow transplantation·2004
Same author

Search for the radiative decays B-->rhogamma and B0-->omegagamma.

Physical review letters·2004
Same author

Measurement of the branching fraction and polarization for the decay B--->D*0K*-.

Physical review letters·2004
Same author

Measurements of the mass and width of the eta(c) meson and of an eta(c)(2S) candidate.

Physical review letters·2004
Same author

Search for lepton-flavor violation in the decay tau- --> l- l+ l-.

Physical review letters·2004

Area of Science:

  • Biochemistry
  • Structural Biology
  • Crystallography

Background:

  • Human C-reactive protein (CRP) is a key inflammatory marker.
  • Understanding CRP's structure is crucial for its biological function.
  • Previous studies suggested a pentameric structure for CRP.

Purpose of the Study:

  • To confirm the pentameric structure of human C-reactive protein using crystallographic methods.
  • To investigate the factors affecting the reproducible growth of CRP crystals.
  • To determine the orientation of the CRP pentamer in different crystal forms.

Main Methods:

  • Rotation function analysis of two tetragonal crystal forms of human CRP.
  • Crystallization experiments to identify limiting factors.

Related Experiment Videos

  • Comparison of unit cell parameters and molecular orientation.

    • Main Results:

    • Rotation function studies confirmed the pentameric quaternary structure of human CRP.
    • Two crystal forms (space groups P4122 (I) and P4222 (II)) were identified with similar unit cells.
    • Dissociation heterogeneity was identified as a major obstacle for reproducible single crystal growth.
    • The orientation of the CRP pentamer was found to be nearly identical in both crystal forms.

    Conclusions:

    • The pentameric structure of human C-reactive protein is definitively confirmed.
    • Strategies to overcome dissociation heterogeneity are needed for improved crystal growth.
    • Consistent molecular orientation provides a basis for further structural studies of CRP.