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Antiproliferative Properties of Clausine-B against Cancer Cell Lines
Wan Nor I'zzah Wan Mohd Zain1, Asmah Rahmat, Fauziah Othman
1Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Background:
CLAUSINE B, A CARBAZOLE ALKALOID ISOLATED FROM THE STEM BARK OF CLAUSENA EXCAVATA, WAS INVESTIGATED FOR ITS ANTIPROLIFERATIVE ACTIVITIES AGAINST FIVE HUMAN CANCER CELL LINES: HepG2 (hepatic cancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breast cancer), HeLa (cervical cancer), and CAOV3 (ovarian cancer).
Methods:
Chang liver (normal cells) was used as a control. The effect of clausine-B was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.
Results:
Clausine-B was found to be active (IC(50)<30 μg/mL) against four of the cancer cell lines tested. The IC(50) values for these four lines were: 21.50 μg/mL (MDA-MB-231), 22.90 g/ml (HeLa), 27.00 μg/mL (CAOV3) and 28.94 μg/mL (HepG2). Clausine-B inhibited the MCF-7 cancer cell line at 52.90 μg/mL, and no IC(50) value was obtained against Chang liver.
Conclusion:
It is possible that the phenolic group in clausine-B responsible for the antiproliferative activities found in this study.
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